HPLC analyses were done using a C18 column on standard lycopene and samples obtained by extraction from tomato paste with different combinations of solvents. Two different combinations of mobile phases for HPLC were initially used: 42:42:16(v/v/v) acetonitrile, methanol and dichloromethane; and 3:1 (v/v) acetonitrile and dichloromethane. The UV-Vis spectra for lycopene gave a maximum adsorption at 472 and 480 nm, which is the same as reported by Davis et al. (2003) and Roh et al. (2013).