The second trial was done using a method developed by Motilva et al. (2014) to extract lycopene and other carotenoids from tomato paste using a tri-mixture of
(a)
(b)
Upper organic layer contains Lycopene
Lower aqueous layer contains water and acetone
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50:25:25 (v/v/v) hexane: acetone: ethanol. The tomato paste became light in colour after a 20-min, single step stirred extraction, indicating that the carotenoids had been extracted. The solution was separated into two layers in a separating funnel (Figure 4-2). The upper layer contained lycopene and carotenoids (β-carotene) which were further separated using a silica column (Figure 4-3) and thin layer chromatography.
Figure 4-